PhD Position LIED (m/f/d)

Pemphigoid diseases (PD) are a heterogeneous group of subepidermal blistering autoimmune skin diseases characterized by blistering on the skin and mucous membranes and autoantibodies against structural proteins of the dermal-epidermal junction. Previously, we have developed a human skin organ culture model for pemphigus vulgaris. Human skin is placed into plates and is cultured in semi-submerged conditions. To induce experimental pemphigus, a single chain variable fragment binding to both desmoglein 1 and 3 is injected into the cultured skin. This procedure consistently induces intraepidermal split formation.

The aim of the project is to apply this method to obtain comprehensive insights into Dsg1/3 autoantibody induced signaling in skin and mucosal keratinocytes. Identified kinases will be validated across several model systems including human skin/mucosa organ culture models. In addition to these biological insights, we also try to identify novel therapeutic targets for the treatment of pemphigus that have the potential to significantly shorten the time to remission.

We are looking for a that is highly motivated candidate and would like to join our team. The position is to be filled as soon as possible and is initially limited until 31.08.2025.

What we offer:

• The salary will be based on the German E 13 TV-L scale, if terms and conditions under collective bargaining law are fulfilled
• Weekly working time is 50 % of a full-time position (currently 19,25 hours per week)
• Individualized supervision and mentoring
• Excellent research environment

Your tasks:

The work will involve protein chemistry including molecular biology, expression and purification of proteins, RT-PCR, immunohistochemistry, direct and indirect immunofluorescence microscopy and cell culture.The position is integrated in a team of PhD students, undergraduate students and technicians that all work on different aspects of autoimmune blistering diseases. You will work in the Division Visualisation (Director, Prof. Jennifer Hundt, DVM, MD, PhD) of the Lübeck Institute of Experimental Dermatology (LIED), University of Lübeck. The position will be integrated in the DFG-funded Research Training Group 2633 Defining and Targeting Autoimmune Pre-Diseaseincluding structured mentoring, teaching, and skills training.

Your profil:

• PhD or master in lifescience
• Self-motivated, creative and detail-oriented with excellent problem-solving skills
• Have teamwork competency but can also work highly independently
• Preferably have immunology experience
• Preferably have experience with organ culture models
• Have good written and oral communication skills in English
• Have a strong passion for science

We are looking forward to your complete application documents, including CV, a cover letter outlining your qualifications for this position and a list of your methods and techniques. Please submit your application until02.05.2023, indicating your earliest possible starting date as well as the reference number 17821.